Composite

Part:BBa_J34200:Design

Designed by: ETH Zurich 2006 iGEM team   Group: iGEM06_ETHZ   (2006-08-15)


XOR gate, 2x PoPS input, 1x PoPS output


Assembly Compatibility:
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    Illegal SpeI site found at 4458
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    Illegal PstI site found at 4908
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    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 15
    Illegal EcoRI site found at 1789
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    INCOMPATIBLE WITH RFC[21]
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    INCOMPATIBLE WITH RFC[23]
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    Illegal EcoRI site found at 1789
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    Illegal XbaI site found at 27
    Illegal XbaI site found at 2172
    Illegal SpeI site found at 2064
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    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 15
    Illegal EcoRI site found at 1789
    Illegal EcoRI site found at 2502
    Illegal EcoRI site found at 4884
    Illegal XbaI site found at 27
    Illegal XbaI site found at 2172
    Illegal SpeI site found at 2064
    Illegal SpeI site found at 4458
    Illegal PstI site found at 1816
    Illegal PstI site found at 4908
    Illegal NgoMIV site found at 3796
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  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4392
    Illegal SapI.rc site found at 4740


Design Notes

The initial promoters are omitted, so that the light sensing system can accept PoPs from another device, and the promoter on the end produces PoPs from this device

I lambda repressor fused via TEV cleavage site to alpha subunit of RNAP NTD with 1 RBS (strong)

cI lambda repressor fused via TVMV cleavage site to alpha subunit of RNAP NTD with 1 RBS (strong)

Source

We used here two specific sequences for cleavage by TEV and TVMV proteases.

References

Sreedevi Nallamsetty et al., Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro, Protein Expression and Purification 38 (2004) 108–115

Rachel B. Kapust and David S. Waughn, Controlled Intracellular Processing of Fusion Proteins by TEV Protease, Protein Expression and Purification 19, 312–318 (2000)